The Influence of Extenders on Motility Duration of Gray Mullet (Mugil cephalus) Spermatozoa

The goal in this study, was to monitor motility duration of Mugil cephalus spermatozoa in different extenders . Fifteen extenders were used in the study which include: Coelomic fluid of four different concentrations (nondiluted, 0.1%, 1% and 10%) BSA(Bovine Serum Albumin) with two concentrations (10mg/ml, 30mg/ml diluted in distilled water), Two saline extenders: 1. (45mMNaCI,5mMKCI, 2.5mMTris, 19mMGlycin, pHs), 2. (500mMNaCI, 3.1mMKCI, 0.2mMTris, 3.4mMCaCh,pH7.5) Two solutions of NaCI(50mM, 250mM)that were used following centrifuging of milt and separating of spermatozoa. These solutions were used to replaced seminal fluid .
Fertilizing fluid with 5 different salinities of 10, 15, 20, and 25ppt along with 32 ppt, used as control.
The duration of sperm motility varied widely in different extenders. Spermatozoa were inactive in coelomic fluid of different concentrations, in either of BSA,saline extender numberl , both solutions of NaCI as well as fertilizing fluid with salinity of 10ppt.
Mean duration of sperm motility ranged between 77.6-149.3, 26.66-68 .6, 43.33-132.3, 59-135.3, and 73.33-138 seconds in saline extender 2, fertilizing fluid with salinities of 15,20,25,32 (control) ppt respectively. The longest duration of sperm motility was achieved in saline extender number 2 namely (500mMNaCI, 3.1 r!'-lVfJ(CI, 0.2mMTris, 3.4mMCaCh, pH7.s).Results Finally indicated that M cephalus spermatozoa are active in hyperosmotic solutions.